SUPPORT OUR MISSION Help us create more free educational content! Donate via PayPal Giving Fund: The Sequel IIe System produces highly accurate long reads (HiFi reads) quickly and cost-effectively thanks to increased
The PacBio Kinnex kits are based on the MAS-Seq method, where smaller amplicons (e.g. 16S rRNA, eukaryotic transcripts) are HiFi sequencing allow you to accurately detect all types of variants, from single nucleotides to structural variants, with high precision, recall, and in-phase
PacBio HiFi Sequencing - CD Genomics How can HiFi long-read sequencing achieve cost parity with short reads?* In this PacBio PRISM 2025 presentation, Mark Van
A decade of HiFi: Implementing long-read sequencing for clinical genomics PacBio HiFi sequencing has been used to generate the latest and most complete version of the human genome, characterize
How HiFi sequencing works - PacBio In this talk, speakers provide an understanding HiFi sequencing methods for resolving viral diversity in complex systems, Go beyond the standard reference to see a more complete picture.* This film explores how leading researchers are using HiFi
In this video, Dave Miller from PacBio and Alvaro Hernandez PhD from the University of Illinois Urbana-Champaign discuss how PacBio is the only sequencing technology to offer HiFi reads that provide accuracy of 99.9%, on par with short reads and Sanger sequencing.
Improving antibody discovery with HiFi long-read sequencing Through Pharmacogenomics (PGx), we can explore how a person's genome affects their response to drugs to enable the
In this webinar hear from Mayo Clinic scientist, Evan Udine, on using the PureTarget and Kinnex kits, alongside other PacBio Long and Accurate: How HiFi Sequencing is Transforming Genomics How do you translate a powerful research technology into a robust, routine clinical tool? At PacBio PRISM 2025, Stuart Scott, PhD,
In this PacBio Virtual Global Summit 2020 presentation, Nicole Newell of PacBio provides an overview of the library preparation With HiFi Sequencing from PacBio you get the benefits of short reads and traditional long reads in one easy-to-use technology. With HiFi sequence reads, DNA templates of 10–30 kb in length are subjected to circular consensus sequencing (CCS) allowing to derive a
Dr. Rob Edwards from San Diego State University describes Pacific Biosciences SMRT single-molecule long read sequencing. Introduction to PacBio Highly Accurate Long-Read Sequencing Macrogen Europe: Pacbio is another member of The House of Sequencing. In this clip, you
Comprehensive de novo mutation discovery with HiFi long-read At PacBio PRISM, Boston, Dr. Midhat Farooqi of Children's Mercy Kansas City discusses using HiFi sequencing to improve the
Application Tutorial: Introduction to Targeted Sequencing with HiFi Reads Learn from leading clinical research laboratories how they are validating and adopting PacBio HiFi long-read sequencing to HiFi sequencing & immune diversity in vaccine response
Advancing Pharmacogenomics Research and the Need for Highly Accurate Long-Read Sequencing Seminar Abstract: Today's most popular DNA sequencing platforms produce reads that are 300 bp long with base accuracy of The PacBio HiFi sequencing method yields highly accurate long-read sequencing datasets with read lengths averaging 10-25 kb and accuracies greater than 99.5%.
Watch this short video to learn about highly accurate long-read sequencing and how HiFi reads can advance scientific discovery. Recent developments in PacBio high-fidelity (HiFi) sequencing technologies have transformed genomic research, with circular consensus HiFi sequencing is a single-molecule, real-time sequencing technology (SMRT) that provides incredible single-molecule read accuracy across long reads.
Highly accurate long-read HiFi sequencing data for five complex Pacific Biosciences Sequencing In this PacBio User Group Meeting presentation, PacBio scientist Meredith Ashby shared several examples of analysis — from
0:00 -Alex Robertson - HiFi Solves, a global consortium of clinical genomics researchers (brief introduction and updates) 2:45 How does HiFi long-read sequencing provide a clearer view of the human microbiome?* In this webinar, see how HiFi reads
Exploring the potential clinical utility of HiFi sequencing for homologous loci (pseudogenes) In this webinar, learn about the sequencing technology that is pushing neuroscience research forward, particularly in Parkinson's
PacBio workshop—ASHG 2024: Integrating HiFi sequencing into clinical practice & population genomics PacBio R&D roadmap to high-throughput HiFi sequencing (PRISM 2025) In this PacBio Virtual Global Summit 2020 presentation, Meredith Ashby of PacBio shares how researchers are using PacBio HiFi
Technical Tutorial: Choosing the best library prep for HiFi sequencing PacBio | About HiFi sequencing, multiplexing and Kinnex kit
Gene therapy is at an inflection point and experiencing tremendous growth. High accuracy and complete visibility are critical to the Advancing human health with HiFi long-read sequencing DNA and Sample Preparation for PacBio HiFi Sequencing - Best Practices and FAQ
Discover the Vega System from PacBio—delivering trusted HiFi long-read sequencing to labs of all sizes. Accessible and Title: "PacBio HiFi Sequencing, DNA Methylation, and Single-cell MAS-ISO Sequencing for Cancer Research" Speaker: Inna
As the first video in our series on PureTarget, this presentation explains the core principles of PacBio HiFi sequencing—the Streamlined chromosome-scale phasing with HiFi sequencing HiFi Sequencing: PacBio's Breakthrough in Detecting Genetic Variants
HiFi Reads - Highly accurate long-read sequencing - PacBio Overview of Illumina Sequencing by Synthesis Workflow | Standard SBS chemistry
Use of HiFi sequencing for structural variant detection in pediatric cancer HiFi Sequencing | Highly Accurate Long Read Sequencing | PacBio HiFi Reads | PacBio Sequencing |
Enhance antibody development workflows by capturing full-length, paired variable regions with HiFi sequencing.* This webinar PacBio Data Deep Dive: A Closer Look at HiFi Sequencing KAPA HiFi HotStart ReadyMix - Roche Sequencing Store
PacBio Sequencing and software enable the generation of highly accurate (99.9%) long reads. HiFi reads are accurate, essential, Precision immunogenic profiling in cancer with HiFi sequencing
Implementing long-read HiFi sequencing: Amplicon, panel, and genome sequencing | Dr. Stuart Scott Understanding PacBio HiFi Sequencing
Highly accurate HiFi reads for discovery, design, and manufacturing of AAV gene therapy Watch this short tutorial to learn how to get started with the full-length RNA sequencing SMRT Sequencing application using PacBio Sequencing is powered by Single Molecule, Real-Time (SMRT) Sequencing technology. The Sequel II System offers the
HiFi sequencing, genetic variants, Pacific Biosciences, genomic research, variant detection Discover how Pacific Biosciences' Application Tutorial: Introduction to Full-Length RNA Sequencing with HiFi Reads
Welcome to our 2024 ASHG workshop! In this session, our speakers introduce a new era in rare disease research, powered by HiFi sequencing enables simultaneous calling of the four DNA bases and 5-methylcytosine (5mC) from untreated genomic DNA, allowing for genome-wide detection and
PacBio HiFi Sequencing, DNA Methylation, and Single-cell MAS-ISO Sequencing for Cancer Research Unlocking human microbiome clarity with PacBio HiFi sequencing Explore the Illumina next-generation sequencing workflow, including sequencing by synthesis (SBS) technology, in 3-dimensional
Kinnex explained—how concatenating smaller amplicons increases throughput for PacBio HiFi sequencing The PacBio HiFi sequencing method yields highly accurate long-read sequencing datasets with read lengths averaging 10–25 kb and accuracies greater than 99.5%.
PacBio sequencing instruments are powered by Single Molecule, Real-Time (SMRT) Sequencing technology. Learn how to go Meet the new Vega System from PacBio: HiFi sequencing within reach
Characterizing C9orf72 repeat expansions and transcripts using HiFi sequencing Understanding the Biology of Genomes with HiFi Sequencing
HiFi on the brain – advancing Parkinson's disease research using HiFi sequencing The Long and Short of Sequencing - Why HiFi Reads are the Future In this PAGBioDay 2021 presentation, Medicinal Genomics CEO Kevin McKernan utilizes HiFi sequencing and genetic maps to
In this CSHL Biology of Genomes 2021 virtual workshop, Jonas Korlach from PacBio discusses examples of how advances in Resolving Viral Evolution and Quasispecies Diversity with HiFi Sequencing
Computational Advances in Genome and Transcriptome Using HiFi Sequencing Children's Mercy First to Use 5-Base HiFi Genomic Sequencing in
With highly accurate long reads (HiFi reads) of greater than 99% accuracy, the Sequel IIe System is the tool of choice for HiFi Sequencing - Unlock Your Next Great Discovery Beyond Short Reads: How HiFi Sequencing is Shaping the Future of Genomic Studies
Library preparation and HiFi sequencing applications for AAV Unbiased, Efficient Characterization of Metagenome Functions with PacBio HiFi Seque
Reconstructing complete phased genomes is key to understanding the genetic basis of many complex traits. However, the Discover the Power of PacBio's HiFi Sequencing Technology for Multi-Omic Approaches. In the last few years, significant
In this webinar, Edward Oakeley, PhD, Novartis, shares and explains his experience and the developments of the HiFi AAV Melissa Smith, PhD, Assistant Professor at University of Louisville speaks at PacBio Prism in Bangkok.
Metagenome Composition and Function Using HiFi Sequencing on the Sequel II System It is the first health care system to use 5-base HiFi sequencing, the world's most-advanced genomic sequencing technology from PacBio, in the clinical setting.
At PacBio PRISM, Melissa Smith, CEO of Clareo Biosciences, explains how HiFi sequencing is essential for understanding the Implementation of HiFi sequencing: insights from clinical laboratories
HiFi Sequencing Resolving Repetitive Cannabis Genomes Watch this short tutorial to learn how to get started with the Targeted sequencing application using highly accurate long reads In this talk, Dr. Wenger describes how whole-genome sequencing (WGS) with accurate, long HiFi reads identify all the variations
The utility of new highly accurate long reads, or HiFi reads, was first demonstrated for calling all variant types in human genomes. Introduction to Highly Accurate Long-Read Sequencing (HiFi Sequencing)
TSS: PacBio presents the Revio and Onso systems for HiFi and sequencing by binding (SBB) sequencing The Present and Future of HiFi Whole-Genome Sequencing for Rare Disease
The HiFi sequencing advantage for metagenome assembly Unlocking the Potential of Multi-Omic Research with PacBio's Advanced HiFi Sequencing Technology HiFi Sequencing for Understanding the Biology of Genomes
PacBio Sequencing – How it Works KAPA HiFi HotStart is designed for routine, high-fidelity PCR of a wide range of targets and fragment sizes. It offers error rates approximately 100 times
Creating Core Demand with HiFi Sequencing Why do some cancer patients respond well to immunotherapy while others suffer severe side effects? Traditional immune profiling Stuart A. Scott, PhD, FACMG from Stanford University speaks at PacBio Prism in Houston.